Affordable Access

deepdyve-link
Publisher Website

Coffee husk composting: an investigation of the process using molecular and non-molecular tools.

Authors
  • Shemekite, Fekadu1
  • Gómez-Brandón, María2
  • Franke-Whittle, Ingrid H2
  • Praehauser, Barbara2
  • Insam, Heribert2
  • Assefa, Fassil3
  • 1 Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia. Electronic address: [email protected] , (Ethiopia)
  • 2 Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, A-6020 Innsbruck, Austria. , (Austria)
  • 3 Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia. , (Ethiopia)
Type
Published Article
Journal
Waste management (New York, N.Y.)
Publication Date
Mar 01, 2014
Volume
34
Issue
3
Pages
642–652
Identifiers
DOI: 10.1016/j.wasman.2013.11.010
PMID: 24369846
Source
Medline
Keywords
License
Unknown

Abstract

Various parameters were measured during a 90-day composting process of coffee husk with cow dung (Pile 1), with fruit/vegetable wastes (Pile 2) and coffee husk alone (Pile 3). Samples were collected on days 0, 32 and 90 for chemical and microbiological analyses. C/N ratios of Piles 1 and 2 decreased significantly over the 90 days. The highest bacterial counts at the start of the process and highest actinobacterial counts at the end of the process (Piles 1 and 2) indicated microbial succession with concomitant production of compost relevant enzymes. Denaturing gradient gel electrophoresis of rDNA and COMPOCHIP microarray analysis indicated distinctive community shifts during the composting process, with day 0 samples clustering separately from the 32 and 90-day samples. This study, using a multi-parameter approach, has revealed differences in quality and species diversity of the three composts.

Report this publication

Statistics

Seen <100 times