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Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes.

Authors
  • Sharp, P M
  • Tuohy, T M
  • Mosurski, K R
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jul 11, 1986
Volume
14
Issue
13
Pages
5125–5143
Identifiers
PMID: 3526280
Source
Medline
License
Unknown

Abstract

Codon usage data has been compiled for 110 yeast genes. Cluster analysis on relative synonymous codon usage revealed two distinct groups of genes. One group corresponds to highly expressed genes, and has much more extreme synonymous codon preference. The pattern of codon usage observed is consistent with that expected if a need to match abundant tRNAs, and intermediacy of tRNA-mRNA interaction energies are important selective constraints. Thus codon usage in the highly expressed group shows a higher correlation with tRNA abundance, a greater degree of third base pyrimidine bias, and a lesser tendency to the A+T richness which is characteristic of the yeast genome. The cluster analysis can be used to predict the likely level of gene expression of any gene, and identifies the pattern of codon usage likely to yield optimal gene expression in yeast.

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