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CO-binding c-type cytochromes and a high-potential cytochrome c in Nitrosomonas europaea.

Authors
  • Miller, D J
  • Wood, P M
Type
Published Article
Journal
The Biochemical journal
Publication Date
May 01, 1983
Volume
211
Issue
2
Pages
503–506
Identifiers
PMID: 6307283
Source
Medline
License
Unknown

Abstract

The purification of two soluble CO-binding cytochromes c from Nitrosomonas europaea is described. Cytochrome cCO-550 ran on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with an apparent Mr of 32 000, whereas for cytochrome cCO-552 the apparent Mr was 16 000. Redox potentials (Em, 7) were determined as +140 and -50mV respectively. Cytochrome cCO-550 was co-purified with a cytochrome c-553, for which an unusually high redox potential of +450mV was measured. These latter components were not resolved by gel-filtration chromatography or electrophoresis under denaturing conditions.

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