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Cloning and sequencing of the cDNA encoding beta-glucosidase 1 from Aspergillus aculeatus.

Authors
  • Kawaguchi, T
  • Enoki, T
  • Tsurumaki, S
  • Sumitani, J
  • Ueda, M
  • Ooi, T
  • Arai, M
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Sep 16, 1996
Volume
173
Issue
2
Pages
287–288
Identifiers
PMID: 8964516
Source
Medline
License
Unknown

Abstract

A cDNA was isolated from an Aspergillus aculeatus cDNA library using synthetic oligodeoxyribonucleotide mixtures that corresponded to the internal amino acid (aa) sequence of mature beta-glucosidase 1 (BGL1). Analysis of the nucleotide sequence of the cloned cDNA insert revealed a 2580-bp open reading frame (ORF) that encoded a 860-aa protein. The deduced aa sequence of the ORF shared sequence similarity with several BGL from other microorganisms.

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