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Cloning of the pfaP gene of Leptospira borgpetersenii.

Authors
  • Trueba, G A
  • Bolin, C A
  • Zuerner, R L
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Jul 04, 1995
Volume
160
Issue
1
Pages
133–134
Identifiers
PMID: 7628708
Source
Medline
License
Unknown

Abstract

A lambda gt11 library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (lambda F15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

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