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Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus.

Authors
  • Perilli, M
  • Franceschini, N
  • Segatore, B
  • Amicosante, G
  • Oratore, A
  • Duez, C
  • Joris, B
  • Frère, J M
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
Sep 15, 1991
Volume
67
Issue
1
Pages
79–84
Identifiers
PMID: 1778425
Source
Medline
License
Unknown

Abstract

The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase.

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