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Cloning of maximakinin precursor cDNAs from Chinese toad, Bombina maxima, venom.

Authors
Type
Published Article
Journal
Peptides
0196-9781
Publisher
Elsevier
Publication Date
Volume
24
Issue
6
Pages
853–861
Identifiers
PMID: 12948837
Source
Medline
License
Unknown

Abstract

Using a novel technique that we have developed for cloning of amphibian skin secretion peptide cDNAs from lyophilized samples, we report here that maximakinin (DLPKINRKGP-bradykinin) is encoded by two different cDNAs, named BMK-1 and BMK-2, containing either four tandem repeat sequences or a single copy. The open reading frames of both precursor cDNAs were found to be 152 and 116 amino acid residues, respectively. These data provide evidence that the structural diversity of peptides in amphibian skin secretions arising from molecular evolutionary events, can be mediated by parallel diversity in encoding mRNAs that in itself may reflect serial gene duplications.

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