Affordable Access

Cloning, expression and evaluation of a recombinant sub-unit vaccine against Clostridium botulinum type F toxin.

Authors
Type
Published Article
Journal
Vaccine
0264-410X
Publisher
Elsevier
Publication Date
Volume
19
Issue
2-3
Pages
288–297
Identifiers
PMID: 10930684
Source
Medline

Abstract

A synthetic gene encoding the Hc (binding) domain of Clostridium botulinum neurotoxin F (FHc) was expressed in Escherichia coli fused to maltose binding protein (MBP). The purified MBP-FHc and FHc isolated after removal of MBP were evaluated in mice for their ability to protect against toxin challenge. Balb/c mice developed a protective immune response following administration of either protein via the intraperitoneal or intramuscular routes. A comparison of antibody titres and protection following single and multiple vaccinations and the effects of dosage are shown. The long term protection afforded by the vaccines was also investigated. Ten months following vaccination mice were still protected when challenged with 10(4) MLD(50) doses of botulinum toxin F.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments