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Cloning, expression and characterization of acharan sulfate-degrading heparin lyase II from Bacteroides stercoris HJ-15.

Authors
  • Hyun, Y-J
  • Lee, K-S
  • Kim, D-H
Type
Published Article
Journal
Journal of Applied Microbiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jan 01, 2010
Volume
108
Issue
1
Pages
226–235
Identifiers
DOI: 10.1111/j.1365-2672.2009.04418.x
PMID: 19566715
Source
Medline
License
Unknown

Abstract

This study provides easy and certain means for obtaining large amounts of pure rHepII and also provides important information regarding the tendencies of this enzyme and its digested products. rHepII digests HLGAGs in a different manner than heparinases from Flavobacterium heparinum; therefore, we anticipate that rHepII will be a powerful tool for studies of GAGs and GAGs lyases.

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