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Cloning of DNA fragments from the left end of the adenovirus type 12 genome: transformation by cloned early region 1.

Authors
  • Byrd, P J
  • Chia, W
  • Rigby, P W
  • Gallimore, P H
Type
Published Article
Journal
The Journal of general virology
Publication Date
Jun 01, 1982
Volume
60
Issue
Pt 2
Pages
279–293
Identifiers
PMID: 6286853
Source
Medline
License
Unknown

Abstract

The human adenovirus serotype 12 (Ad-12) EcoRI-C DNA fragment (0 to 16.5 map units) was cloned in the plasmid vector pAT153. This cloned Ad-12 EcoRI-C DNA fragment was subcloned generating recombinant plasmids which contained the Ad-12 SalI-C fragment (0 to 10.3 map units), the Ad-12 HindIII-G fragment (0 to 6 X 8 map units) and the Ad-12 AccI-H fragment (0 to 4 X 7 map units). Thus, we constructed recombinant plasmids which contain Ad-12 DNA sequences which represent all or part of the virus transforming gene region. The capacity of the cloned Ad-12 EcoRI-C DNA fragment to transform rat cells in vitro was assessed using the focus assay on primary cultures of rat cells. The specific transforming activity of this recombinant plasmid was in the same range as that found for intact Ad-12 DNA. Transformed foci which were induced by the cloned Ad-12 EcoRI-C DNA fragment were established as cell lines and the presence of Ad-12 DNA in these lines was demonstrated using the Southern blotting technique.

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