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[Cloning of the DNA fragment of a transgenic mouse containing an integrated recombinant plasmid].

Authors
  • Tarantul, V Z
  • Kucheriavyĭ, V V
  • Makarova, I V
  • Baranov, Iu N
  • Begetova, T V
Type
Published Article
Journal
Molekuliarnaia biologiia
Publication Date
Jan 01, 1986
Volume
20
Issue
1
Pages
278–287
Identifiers
PMID: 3005843
Source
Medline
License
Unknown

Abstract

The "plasmid rescue" method has been used to isolate the recombinant plasmid pMA3 fragment and flanking sequences from the transgenic mouse genome containing the fragment in integrated form. The "rescued" plasmid, pMAR1, lacks all virus sequences and retains only those regions of pBR322 that are responsible for the plasmid replication and Escherichia coli ampicillin resistance. The plasmid pMA3 deletion has occurred at its integration into the mouse genome after microinjection into the zygote. The integrated fragment of the plasmid is adjacent to the genome repeated sequence that is highly conservative in evolution.

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