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Cloning of cDNAs encoding a rat neuropeptide immunologically related to salmon melanin-concentrating hormone.

Authors
  • Fellmann, D
  • Bresson, J L
  • Breton, C
  • Bahjaoui, M
  • Rouillon, A
  • Gouget, A
  • Bugnon, C
Type
Published Article
Journal
Neuroscience Letters
Publisher
Elsevier
Publication Date
Nov 20, 1989
Volume
106
Issue
1-2
Pages
23–28
Identifiers
PMID: 2586828
Source
Medline
License
Unknown

Abstract

In order to identify the neuropeptide related to melanin-concentrating hormone (MCH) synthetized by neurons of the posterior hypothalamus in the mammals, we have screened rat hypothalamus and rat brain cDNA expression libraries using MCH antiserum. We isolated 5 distinct immunopositive recombinants with cross-hybridizing cDNA inserts. One of them hybridized to RNAs exclusively located in neurons stained by the same antiserum, as seen by successively performing in situ hybridization and then an immunocytochemical technique on the same section. Sequencing of this MCH-like cDNA is in progress.

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