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Cloning and Sequencing of an Alkaline Protease Gene from Bacillus lentus and Amplification of the Gene on the B. lentus Chromosome by an Improved Technique

Authors
  • Per Linå Jørgensen
  • Martin Tangney
  • Poul Erik Pedersen
  • Sven Hastrup
  • Børge Diderichsen
  • Steen T. Jørgensen
Publisher
American Society for Microbiology
Publication Date
Feb 01, 2000
Source
PMC
Keywords
License
Unknown

Abstract

A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

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