Affordable Access

Clonidine and phenylephrine injected into the lateral preoptic area reduce water intake in dehydrated rats.

Authors
  • Callera, J C
  • Camargo, L A
  • De Luca Júnior, L A
  • Menani, J V
  • Renzi, A
  • Saad, W A
Type
Published Article
Journal
Pharmacology Biochemistry and Behavior
Publisher
Elsevier
Publication Date
Sep 01, 1993
Volume
46
Issue
1
Pages
39–43
Identifiers
PMID: 7902984
Source
Medline
License
Unknown

Abstract

In the present study, we investigated the effect of phenylephrine and clonidine (alpha 1- and alpha 2-adrenoceptor agonists, respectively) injected into the lateral preoptic area (LPOA) on the water intake induced by water deprivation in rats. In addition, the effects of prior injections of prazosin and yohimbine (alpha 1- and alpha 2-adrenoceptor antagonists, respectively) into the LPOA on the antidipsogenic action of phenylephrine and clonidine were investigated. After 30 h of water deprivation, the water intake of rats in a control experiment (saline injection) was 10.5 +/- 0.8 ml/h. Injection of clonidine (5, 10, 20, and 40 nmol) into the LPOA reduced water intake to 6.3 +/- 0.9, 4.9 +/- 0.8, 3.6 +/- 1.0, and 2.2 +/- 0.7 ml/h, respectively. Similar reductions occurred after injection of 80 and 160 nmol phenylephrine into the LPOA (6.2 +/- 1.6 and 4.8 +/- 1.3 ml/h, respectively). Pretreatment with prazosin (40 nmol) abolished the antidipsogenic action of an 80-nmol dose of phenylephrine (11.3 +/- 1.1 ml/h) and reduced the effect of a 20-nmol dose of clonidine (7.4 +/- 1.4 ml/h). Yohimbine (20, 40, and 80 nmol), previously injected, produced no significant changes in the effects of either phenylephrine or clonidine. The present results show that phenylephrine and clonidine injected into the LPOA induce an antidipsogenic effect in water-deprived rat. They also suggest an involvement of alpha 1-adrenoceptors in this effect. A possible participation of imidazole receptors in the effect of clonidine should also be taken into account.

Report this publication

Statistics

Seen <100 times