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A Clinical Trial to Evaluate the Efficacy of α-Viniferin in Staphylococcus aureus – Specific Decolonization without Depleting the Normal Microbiota of Nares

Authors
  • RAHIM, MD ABDUR1, 2
  • SEO, HOONHEE2
  • KIM, SUKYUNG2
  • JEONG, YOON KYOUNG2
  • TAJDOZIAN, HANIEH1, 2
  • KIM, MIJUNG2
  • LEE, SAEBIM2
  • SONG, HO-YEON1, 2
  • 1 Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan
  • 2 Probiotics Microbiome Convergence Center, Asan
Type
Published Article
Journal
Polish Journal of Microbiology
Publisher
Exeley Inc.
Publication Date
Mar 12, 2021
Volume
70
Issue
1
Pages
117–130
Identifiers
DOI: 10.33073/pjm-2021-011
Source
Exeley
Keywords
License
Green

Abstract

Staphylococcus aureus is currently a significant multidrug-resistant bacterium, causing severe healthcare-associated and community-acquired infections worldwide. The current antibiotic regimen against this pathogen is becoming ineffective due to resistance, in addition, they disrupt the normal microbiota. It highlights the urgent need for a pathogen-specific drug with high antibacterial efficacy against S. aureus. α-Viniferin, a bioactive phytochemical compound, has been reported to have excellent anti-Staphylococcus efficacy as a topical agent. However, so far, there were no clinical trials that have been conducted to elucidate its efficacy. The present study aimed to investigate the antibacterial efficacy of α-viniferin against S. aureus in a ten-day clinical trial. Based on the results, α-viniferin showed 50% minimum inhibitory concentrations (MIC50 values) of 7.8 μg/ml in culture broth medium. α-Viniferin was administered in the nares three times a day for ten days using a sterile cotton swab stick. Nasal swab specimens were collected before (0 days) and after finishing the trial (10th day), and then analyzed. In the culture and RT-PCR-based analysis, S. ureus was reduced significantly: 0.01. In addition, 16S ribosomal RNA-based amplicon sequencing analysis showed that S. aureus reduced from 51.03% to 23.99% at the genus level. RNA-seq analysis was also done to gain insights into molecular mechanisms of α-viniferin against S. aureus, which revealed that some gene groups were reduced in 5-fold FC cutoff at two times MIC conditions. The study results demonstrate α-viniferin as a potential S. aureus-specific drug candidate.

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