Affordable Access

Access to the full text

Clinical significance of circulating tumor cell related markers in patients with epithelial ovarian cancer before and after adjuvant chemotherapy

  • Yousefi, Meysam1, 1, 2
  • Rajaie, Sara3
  • Keyvani, Vahideh4
  • Bolandi, Somayeh1
  • Hasanzadeh, Malihe1
  • Pasdar, Alireza1, 1, 5
  • 1 Mashhad University of Medical Sciences, Mashhad, Iran , Mashhad (Iran)
  • 2 Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Ahvaz (Iran)
  • 3 Islamic Azad University, Arsanjan Branch, Arsanjan, Iran , Arsanjan (Iran)
  • 4 Shahid Chamran University of Ahvaz, Ahvaz, Iran , Ahvaz (Iran)
  • 5 University of Aberdeen, Foresterhill, Aberdeen, UK , Foresterhill, Aberdeen (United Kingdom)
Published Article
Scientific Reports
Springer Nature
Publication Date
May 18, 2021
DOI: 10.1038/s41598-021-88780-w
Springer Nature


Circulating tumor cells (CTCs) have recently been considered as new prognostic and diagnostic markers for various human cancers; however, their significance in epithelial ovarian cancer (EOC) remains to be elucidated. In this study, using quantitative real-time PCR, we evaluated the expression of EPCAM, MUC1, CEA, HE4 and CA125 mRNAs, as putative markers of CTCs, in the blood of 51 EOC patients before and/or after adjuvant chemotherapy. Our results demonstrated that, before chemotherapy, the expression of EPCAM, MUC1, CEA and HE4 mRNAs were correlated to each other. CEA expression was correlated with tumor stage (r = 0.594, p = 0.000) before chemotherapy, whereas its expression after chemotherapy was correlated with serum levels of CA125 antigen (r = 0.658, p = 0.000). HE4 mRNA showed the highest sensitivity both before and after chemotherapy (82.98% and 85.19%, respectively) and the persistence of this marker after chemotherapy was associated with advanced disease stage. The expression of CA125 mRNA had negative correlation with the other markers and with tumor stage and therapy response (evaluated by the measurement of serum CA125 antigen). Collectively, our results indicated a better clinical significance of tumor-specific markers (CEA and HE4 mRNAs) compared to epithelial-specific markers (EPCAM and MUC1 mRNAs).

Report this publication


Seen <100 times