Objective: To provide evidence for a rational and effective prevention and treatment of Pseudomonas aeruginosa, the clinical characteristics and the resistance to various antibiotics of were investigated.Methods：A retrospective analysis of 224 strains of Pseudomonas aeruginosa isolated from various specimens from various clinical departments of our hospital (April 1, 2018 to June 31, 2019) were conducted. Identification and drug susceptibility test of isolated strains was performed using a fully automatic bacterial identification analyzer (MicroScan WalkAway-96 plus), and data analysis was performed using WH0NET5.6 software. Result：Among all the bacteria isolated in our hospital during the above period, Pseudomonas aeruginosa accounted for 10.09% of them all and 12.57% of Gram-negative bacilli, respectively. These isolates were mainly derived from sputum specimens (68.75%), mainly from male patients (70.54%), and mostly 61-70 (27.23%) or 51-60 (22.77%) years old. Pseudomonas aeruginosa isolates are mainly from Rehabilitation Ward, ICU, and Liver Transplantation Unit, accounted for 29.91%, 12.95% and 10.27% of all isolates, respectively. The sensitivity of Pseudomonas aeruginosa to various antibacterial drugs, in the order of high to low were carbapenems, aztreonam, quinolones, cephalosporins, piperacillin/ tazobactam, aminoglycoside, with a lowest resistance rate (2.4%) to amikacin and a highest resistance rate to imipenem (33.0%). Conclusion：The isolation rate of Pseudomonas aeruginosa was relatively stable during the study period, and among all the P. aeruginosa detected, most of them were from the respiratory secretions of elderly male patients. The resistance rate of Pseudomonas aeruginosa isolates to various antibiotics is mainly within 30%. Clinical units such as Rehabilitation Ward, ICU, and Liver Transplantation Unit have a high detection rate, therefore, these departments should be monitored in a focused manner. Our research provide a scientific basis for the rational use of antibiotics and a better control of Pseudomonas aeruginosa infection.