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[Cleavage of RNA in hybrid duplexes by E. coli ribonuclease H. II. Substrate properties of nucleotides containing non-nucleotide linkers].

Authors
  • Vorob'ev, P E1
  • Pyshnaia, I A
  • Pyshnyĭ, D V
  • Repkova, M N
  • Ven'iaminov, A G
  • Zenkova, M A
  • Ivanova, E M
  • Scalfi-Happ, C
  • Seliger, H
  • Bonora, G
  • Zarytova, V F
  • 1 Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 630090 Russia. [email protected]
Type
Published Article
Journal
Bioorganicheskaia khimiia
Publication Date
November 2000
Volume
26
Issue
11
Pages
844–851
Identifiers
PMID: 11696895
Source
Medline
License
Unknown

Abstract

The 20-mer bridged oligodeoxynucleotides containing short oligomers joined by the hexamethylenediol and hexaethylene glycol linkers were shown to form complementary DNA/DNA and RNA/DNA complexes whose thermostability depends on the length and number of the nonnucleotide linkers. Hybrid complexes of the bridged oligonucleotides proved to be substrates for the E. coli ribonuclease H. The presence of one-three nonnucleotide linkers in a 20-mer decreased the hydrolysis efficacy only 1.2-1.4-fold. It is the composition of the RNA cleavage products that was influenced the most significantly by the nonnucleotide linkers. RNase H simultaneously hydrolyzed the RNA 3'-ends of each hybrid duplex involving a bridged oligonucleotide. The presence of an inverted 3'-3'-phosphodiester bond at the 3'-end of the oligodeoxyribonucleotide only slightly affected the RNase H activity.

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