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CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell.

Authors
  • Tong, Hui1
  • Zhao, Kai2
  • Wang, Jiangjie1
  • Xu, Hui3
  • Xiao, Jianqi2
  • 1 Department of Neurosurgery, Linyi Central Hospital, Linyi, China. , (China)
  • 2 Department of Neurosurgery, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, Southern Medical University, Qiqihar, China. , (China)
  • 3 Department of Neurosurgery, Lianshui County People's Hospital, the Affiliated Lianshui County People's Hospital of Kangda College of Nanjing Medical University, Huai'an, China. , (China)
Type
Published Article
Journal
The Journal of pharmacy and pharmacology
Publication Date
Jan 01, 2020
Volume
72
Issue
1
Pages
68–75
Identifiers
DOI: 10.1111/jphp.13188
PMID: 31721211
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

MicroRNAs are abundant in eukaryotic cells and play key roles in cancers. Circular RNAs (CircRNAs) served as the competing endogenous RNAs (ceRNAs) in mediating multiple cell processes. This study aims to define the role of CircRNA CircZNF609/miR-134-5p in glioma as well as the underlying regulating mechanism. Relative expression of miR-134-5p, CircZNF609 and BTG-2 mRNA was determined by quantitative real-time PCR. Cell proliferation was analysed by CCK-8 assay. Cell migration was assessed by cell wound scratch assay. The direct regulatory of miR-134-5p on BTG-2 and CircZNF609 was verified by luciferase report gene assay. MiR-134-5p was significantly upregulated in glioma cells. The overexpression of miR-134-5p inhibited cell proliferation and migration of glioma cell U251 and U87. Reversely, knock-down of miR-134-5p enhanced cell proliferation and migration. Both BTG-2 and CircZNF609 are the direct targets of miR-134-5p, and their expression could be negatively regulated by miR-134-5p. CircZNF609 was significantly upregulated in U251 and U87 cells and acted as an oncogene to promote cell proliferation and cell migration of glioma cell U251 and U87. These data proved that CircZNF609 served as a competing RNA to bind miR-134-5p that promoted BTG-2 expression leading to reduced proliferation and migration of glioma cell. © 2019 Royal Pharmaceutical Society.

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