Affordable Access

Access to the full text

The Circulation of Type F Clostridium perfringens among Humans, Sewage, and Ruditapes philippinarum (Asari Clams)

Authors
  • Yanagimoto, Keita1, 2
  • Uematsu, Kosei1
  • Yamagami, Takaya1
  • Haramoto, Eiji3
  • 1 (T.Y.)
  • 2 Environmental and Social System Science Course, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
  • 3 Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
Type
Published Article
Journal
Pathogens
Publisher
MDPI
Publication Date
Aug 18, 2020
Volume
9
Issue
8
Identifiers
DOI: 10.3390/pathogens9080669
PMID: 32824805
PMCID: PMC7459516
Source
PubMed Central
Keywords
License
Green

Abstract

Clostridium perfringens is an important pathogen that is responsible for gastroenteritis; the causative agent for the symptoms is C. perfringens enterotoxin (CPE), which is mainly produced by type F C. perfringens . Since shellfishes may gather C. perfringens in the water environment, this study estimated the potential circulation of type F C. perfringens among humans, sewage, and Ruditapes philippinarum (asari clams) as a result of sewage pollution. A comparison of the characteristics among the isolates from 86 sewage influents, 36 effluents, 76 asari clams, and 37 humans was conducted. Serotyping, cpe genotyping, and toxin genotyping showed that C . perfringens with a plasmid IS 1151 sequence downstream of cpe was predominant among sewage influents, effluents, humans, and asari clams. Multilocus sequence typing suggested that some isolates from a human, sewage influents, effluents, and asari clams were linked to each other. These results demonstrated that asari clams are the necessary infection sources of C. perfringens responsible for carriers and foodborne diseases, and that these pathogens from humans infected by asari clams can pollute the water environment. It is useful to assess bacteria such as C. perfringens isolates from sewage to estimate the trend of those from the community.

Report this publication

Statistics

Seen <100 times