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Circulating Exosomes with Distinct Properties during Chronic Lung Allograft Rejection.

Authors
  • Gunasekaran, Muthukumar1
  • Sharma, Monal1
  • Hachem, Ramsey2
  • Bremner, Ross3
  • Smith, Michael A3
  • Mohanakumar, Thalachallour4
  • 1 Norton Thoracic Institute Research Laboratory, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013.
  • 2 Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63110; and.
  • 3 Division of Thoracic Surgery and Lung Transplantation, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013.
  • 4 Norton Thoracic Institute Research Laboratory, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013; [email protected]
Type
Published Article
Journal
The Journal of Immunology
Publisher
The American Association of Immunologists
Publication Date
Feb 28, 2018
Identifiers
DOI: 10.4049/jimmunol.1701587
PMID: 29491008
Source
Medline
License
Unknown

Abstract

Circulating exosomes containing donor HLA and lung-associated self-antigens (SAg) are thought to play an important role in allograft rejection after human lung transplantation. We characterized exosomes isolated from serum of 10 lung transplant recipients (LTxR) diagnosed with bronchiolitis obliterans syndrome (BOS) and compared them with exosomes isolated from serum of 10 stable LTxR. Lung-associated SAg (K-α-1-tubulin [Kα1T] and collagen V [Col-V]), MHC class II molecules, costimulatory molecules CD40, CD80, and CD86, and transcription factors class II MHC trans-activator, NF-κB, hypoxia-inducible factor 1-α, IL-1R-associated kinase 1, MyD88, and 20S proteasome were detected in exosomes from BOS, but not stable LTxR. In contrast, adhesion molecules were present in both groups. C57BL/6 mice immunized with exosomes from BOS but not stable LTxR demonstrated Ab to SAg (Col-V, 33.5 ± 15.7 versus 10.4 ± 6.4, p = 0.021; Kα1T, 925 ± 403 versus 317 ± 285, p = 0.044) and HLA (mean fluorescence intensity: BOS, 8450; stable, 632; p < 0.05). Furthermore, splenic lymphocytes demonstrated increased frequency of lung SAg-specific IL-17 (Col-V, 128 ± 46 versus 31 ± 21, p = 0.013; Kα1T, 194 ± 47 versus 67 ± 43, p = 0.014) and IFN-γ (Col-V, 165 ± 79 versus 38 ± 40, p = 0.042; Kα1T, 232 ± 64 versus 118 ± 39, p = 0.012). Reduced levels of IL-10-producing cells were seen in BOS exosome immunized mice compared with mice immunized with stable exosomes (Col-V, 59 ± 23 versus 211 ± 85, p = 0.016; Kα1T, 78 ± 49 versus 295 ± 104, p = 0.017). Owing to the unique immune-stimulating properties of exosomes induced during rejection, we propose that they play an important role in eliciting both alloantigen- and SAg-specific immunity, leading to chronic rejection after lung transplantation.

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