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Circulating DNA in the blood of gastric cancer patients.

Authors
  • Kolesnikova, Elena V
  • Tamkovich, Svetlana N
  • Bryzgunova, Olga E
  • Shelestyuk, Petr I
  • Permyakova, Valentina I
  • Vlassov, Valentin V
  • Tuzikov, Aleksandr S
  • Laktionov, Pavel P
  • Rykova, Elena Y
Type
Published Article
Journal
Annals of the New York Academy of Sciences
Publisher
Wiley (Blackwell Publishing)
Publication Date
Aug 01, 2008
Volume
1137
Pages
226–231
Identifiers
DOI: 10.1196/annals.1448.009
PMID: 18837952
Source
Medline
License
Unknown

Abstract

The concentration of cell-free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence-based assay and methylation-specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell-surface-bound DNA was not decreased compared with controls and amounted to 80 +/- 15% of the total circulating DNA. MSP analysis of three genes in the cell-surface-bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell-surface-bound DNA is a convenient source of DNA for MSP analysis of cancer-specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer-related changes in DNA can significantly contribute to cancer diagnostics.

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