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CircTHBS1 targeting miR-211/CCND2 pathway to promote cell proliferation and migration potential in primary cystitis glandularis cells.

Authors
  • Ma, Yue1
  • Shan, Zhengfei2
  • Liu, Ying3
  • Shao, Honggang1
  • Xin, Yupeng1
  • He, Kai1
  • Jiang, Shichun1
  • Wang, Yaodong1
  • 1 Department of Urology, Mianyang Central Hospital, Mianyang 621000, Sichuan Province, China. , (China)
  • 2 Department of Organ Transplantation and Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong Province, China. , (China)
  • 3 Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong Province, China. , (China)
Type
Published Article
Journal
Bioscience Reports
Publisher
Portland Press
Publication Date
Aug 27, 2021
Volume
41
Issue
8
Identifiers
DOI: 10.1042/BSR20201164
PMID: 32820798
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The pathogenesis of cystitis glandular (CG) is unclear, but it is generally considered to be a neoplastic lesion of urothelial hyperplasia formed by long-term chronic stimulation. There is growing evidence that circRNAs play important roles in a variety of cellular processes. However, there are few reports on the role and molecular mechanism of circRNA in CG. In the present study, we first isolated primary cells from CG tissues and adjacent normal tissues. Further experiments showed that CircTHBS1 was up-regulated in primary CG cells (pCGs). The results of CCK-8 showed that the overexpression of CircTHBS1 promoted the viability of pCGs, while the deletion of CircTHBS1 reduced the cell viability. Knocking out CircTHBS1 also inhibited the migration of pCGs. In addition, we demonstrated that CircTHBS1 played a role in the adsorption of miR-211 by "sponge" in pCG. In turn, miR-211 can directly target CYCLIN D2 (CCND2) 3'UTR to perform its function. Finally, we confirmed the role and mechanism of CircTHBS1/miR-211/CCND2 regulation axis in pCGs. In summary, our study is the first to reveal the role and underlying mechanism of CircTHBS1 in CG, providing a potential biomarker and therapeutic target for human CG. © 2021 The Author(s).

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