Affordable Access

deepdyve-link
Publisher Website

Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2.

Authors
  • Yoshiba, Satoko
  • Shiratori, Hidetaka
  • Kuo, Ivana Y
  • Kawasumi, Aiko
  • Shinohara, Kyosuke
  • Nonaka, Shigenori
  • Asai, Yasuko
  • Sasaki, Genta
  • Belo, Jose Antonio
  • Sasaki, Hiroshi
  • Nakai, Junichi
  • Dworniczak, Bernd
  • Ehrlich, Barbara E
  • Pennekamp, Petra
  • Hamada, Hiroshi
Type
Published Article
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
Oct 12, 2012
Volume
338
Issue
6104
Pages
226–231
Identifiers
DOI: 10.1126/science.1222538
PMID: 22983710
Source
Medline
License
Unknown

Abstract

Unidirectional fluid flow plays an essential role in the breaking of left-right (L-R) symmetry in mouse embryos, but it has remained unclear how the flow is sensed by the embryo. We report that the Ca(2+) channel Polycystin-2 (Pkd2) is required specifically in the perinodal crown cells for sensing the nodal flow. Examination of mutant forms of Pkd2 shows that the ciliary localization of Pkd2 is essential for correct L-R patterning. Whereas Kif3a mutant embryos, which lack all cilia, failed to respond to an artificial flow, restoration of primary cilia in crown cells rescued the response to the flow. Our results thus suggest that nodal flow is sensed in a manner dependent on Pkd2 by the cilia of crown cells located at the edge of the node.

Report this publication

Statistics

Seen <100 times