Bovine preparations that allow for in vivo measurement of metabolic fluxes across the hindlimb often suffer from limited durability, usually because of failure of the venous catheter. A catheterization procedure that virtually eliminates this occurrence is presented. A silicone rubber catheter is implanted permanently in the femoral vein. It accommodates the repeated insertion and removal at each sampling session of a temporary sampling catheter. A simple and reliable method ensures the positioning of this catheter in the external iliac vein of a conscious, normally standing animal. The application of this approach allowed the study of hindlimb metabolism of cattle for up to 4 mo without a single planned sampling session postponed or missed. This preparation is particularly well suited for studies that require repeated measurements of hindlimb metabolism on the same animals over a period of many months.