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Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides

Authors
  • Xin, Haoyang1
  • Zhang, Tao2
  • Han, Yonghua3
  • Wu, Yufeng4
  • Shi, Jisen1
  • Xi, Mengli1
  • Jiang, Jiming5
  • 1 Nanjing Forestry University, Co-Innovation Center for Sustainable Forestry in Southern China/Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing, 210037, China , Nanjing (China)
  • 2 Yangzhou University, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Centre for Modern Production Technology of Grain Crops/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou, 225009, China , Yangzhou (China)
  • 3 Jiangsu Normal University, The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Xuzhou, 221116, China , Xuzhou (China)
  • 4 Nanjing Agricultural University, The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Bioinformatics Center, Nanjing, 210095, China , Nanjing (China)
  • 5 Michigan State University, Department of Plant Biology, Department of Horticulture, East Lansing, MI, 48824, USA , East Lansing (United States)
Type
Published Article
Journal
Chromosoma
Publisher
Springer-Verlag
Publication Date
Mar 08, 2018
Volume
127
Issue
3
Pages
313–321
Identifiers
DOI: 10.1007/s00412-018-0664-y
Source
Springer Nature
Keywords
License
Yellow

Abstract

Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interestingly, this locus was mapped to either a peritelomeric or a centromeric region among different poplar species. We developed an oligonucleotide (oligo)-based chromosome painting probe based on the sequence of chromosome 19 from Populus trichocarpa. We performed chromosome painting in P. tomentosa and P. deltoides. Surprisingly, the distal end on the short arm of chromosome 19, which corresponds to the location of the sex-determining locus reported in several species, was not painted in both species. Thus, the DNA sequences associated with this region have not been anchored to the current chromosome 19 pseudomolecule, which was confirmed by painting of somatic metaphase chromosome 19 of P. trichocarpa. Interestingly, the unpainted distal ends of the two chromosome 19 did not pair at the pachytene stage in 22–24% of the meiotic cells in the two species, suggest that these regions from the sex chromosomes have structurally diverged from each other, resulting in the reduced pairing frequency. These results shed light on divergence of a pair of young sex chromosomes in poplar.

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