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Chromosome condensation defects in barren RNA-interfered Drosophila cells.

Authors
  • Maria Patrizia Somma
  • Barbara Fasulo
  • Giorgia Siriaco
  • Giovanni Cenci
Publication Date
Nov 01, 2003
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Barren, the Drosophila homolog of XCAP-H, is one of three non-SMC subunits of condensin, a conserved 13S multiprotein complex required for chromosome condensation. Mutations in barren (barr) were originally shown to affect sister-chromatid separation during mitosis 16 of the Drosophila embryo, whereas condensation defects were not detected. In contrast, mutations in yeast homologs of barren result in defective mitotic chromosome condensation as well as irregular chromatid separation. We have used double-stranded RNA-mediated interference (RNAi) to deplete Barren in Drosophila S2 cells. Our analyses indicate that inactivation of barr leads to extensive chromosome condensation and disrupts chromatid segregation.

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