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Chorismate mutase and isochorismatase, two potential effectors of the migratory nematode Hirschmanniella oryzae, increase host susceptibility by manipulating secondary metabolite content of rice.

Authors
  • Bauters, Lander1
  • Kyndt, Tina1
  • De Meyer, Tim2
  • Morreel, Kris3, 4
  • Boerjan, Wout3, 4
  • Lefevere, Hannes1
  • Gheysen, Godelieve1
  • 1 Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. , (Belgium)
  • 2 Department of Data Analysis and Mathematical Modelling, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. , (Belgium)
  • 3 VIB-UGent Center for Plant Systems Biology, Ghent, Belgium. , (Belgium)
  • 4 Department of Plant Biotechnology and Bioinformatics, Faculty of Sciences, Ghent University, Ghent, Belgium. , (Belgium)
Type
Published Article
Journal
Molecular Plant Pathology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Dec 01, 2020
Volume
21
Issue
12
Pages
1634–1646
Identifiers
DOI: 10.1111/mpp.13003
PMID: 33084136
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Hirschmanniella oryzae is one of the most devastating nematodes on rice, leading to substantial yield losses. Effector proteins aid the nematode during the infection process by subduing plant defence responses. In this research we characterized two potential H. oryzae effector proteins, chorismate mutase (HoCM) and isochorismatase (HoICM), and investigated their enzymatic activity and their role in plant immunity. Both HoCM and HoICM proved to be enzymatically active in complementation tests in mutant Escherichia coli strains. Infection success by the migratory nematode H. oryzae was significantly higher in transgenic rice lines constitutively expressing HoCM or HoICM. Expression of HoCM, but not HoICM, increased rice susceptibility against the sedentary nematode Meloidogyne graminicola also. Transcriptome and metabolome analyses indicated reductions in secondary metabolites in the transgenic rice plants expressing the potential nematode effectors. The results presented here demonstrate that both HoCM and HoICM suppress the host immune system and that this may be accomplished by lowering secondary metabolite levels in the plant. © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.

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