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Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis.

Authors
  • Lamberti, Yanina
  • Alvarez Hayes, Jimena
  • Perez Vidakovics, Maria L
  • Rodriguez, Maria Eugenia
Type
Published Article
Journal
FEMS Immunology & Medical Microbiology
Publisher
Oxford University Press
Publication Date
Jul 01, 2009
Volume
56
Issue
2
Pages
143–150
Identifiers
DOI: 10.1111/j.1574-695X.2009.00557.x
PMID: 19385994
Source
Medline
License
Unknown

Abstract

Bordetella pertussis is a re-emerging human respiratory pathogen whose infectious process is not fully understood, hampering the design of effective vaccines. The nature of bacterial attachment to host cells is a key event in the outcome of the infection. However, host cell receptors involved in B. pertussis colonization of the respiratory tract are still under investigation. Here, we report that cholesterol-rich domains are involved in B. pertussis adhesion to epithelial cells. Treatment of A549 cells with cholesterol-sequestering drugs such as methyl-beta-cyclodextrin, nystatin, or filipin resulted in a significant decrease of B. pertussis attachment. Confocal laser microscopy studies showed B. pertussis associated with cholesterol-rich domains. Accordingly, B. pertussis was found in detergent-resistant membrane domain fractions isolated from bacterial-infected A549 cells. Our results indicate a main role of filamentous hemagglutinin, an environmentally regulated virulence factor, in this interaction, and a specific affinity for cholesterol, one of the major components of tracheal secretions, which might additionally contribute to the effective colonization of the respiratory tract.

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