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Chlorinating ability of human phagocytosing leucocytes.

Authors
  • Zgliczyński, J M
  • Stelmaszyńska, T
Type
Published Article
Journal
European journal of biochemistry / FEBS
Publication Date
Aug 01, 1975
Volume
56
Issue
1
Pages
157–162
Identifiers
PMID: 170101
Source
Medline
License
Unknown

Abstract

The course of chlorination in neutrophilic granulocytes has been shown. The process of 36Cl incorporation occurs during and after the engulfment of bacteria by granulocytes. Incorported radioactivity was found in insoluble fractions. The myeloperoxidase obtained from neutrophils catalyzes chlorination of protein (bovine serum albumin) and bacteria (Staphylococcus epidermidis) in the presence of hydrogen peroxide and chloride. The products of chlorination are insoluble. Chlorination in neutrophils is inhibited by the iodide and myeloperoxidase inhibitors azide and cyanide. A quantitative method of determination of biological chlorination in cells has been devised.

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