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Chitosanase activity inBacillus thuringiensis

Authors
  • Cruz Camarillo, R.1
  • Sánchez Pérez, O.1
  • Rojas Avelizapa, N. G.2
  • Gómez Ramírez, M.1
  • Rojas Avelizapa, L. I.1
  • 1 Instituto Politecnico National, Departamento de Microbiologia, Escuela Nacional de Ciencias Biológicas, México, 11340 (DF), México , México
  • 2 Instituto Mexicano del Petróleo, Programa de Biotecnología, México, 07730 (DF), México , México
Type
Published Article
Journal
Folia Microbiologica
Publisher
Springer-Verlag
Volume
49
Issue
1
Identifiers
DOI: 10.1007/BF02931653
Source
Springer Nature
Keywords
License
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Abstract

The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23 %) out of 77 crystalliferous strains ofBacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 °C with shaking in a medium of pH 6.5 with 4 % colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase ofB. thuringiensis.

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