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Chitosan/alginate nanogel potentiate berberine uptake and enhance oxidative stress mediated apoptotic cell death in HepG2 cells.

Authors
  • Singh, Neha1
  • Anand, Sumit Kumar2
  • Sharma, Ankita3
  • Singh, Sukhveer1
  • Kakkar, Poonam4
  • Srivastava, Vikas5
  • 1 CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226 001, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India. , (India)
  • 2 CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226 001, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India; Department of Pathology and Translational Pathobiology, LSU Health, Shreveport, LA-71103, USA. , (India)
  • 3 CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226 001, Uttar Pradesh, India; Department of Biotechnology, National Institute of Pharmaceutical Education and Research-Raebareli, Bijnor-Sisendi Road, Post Office Mati, Lucknow 226002, India. , (India)
  • 4 CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226 001, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India. Electronic address: [email protected]. , (India)
  • 5 CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226 001, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India. Electronic address: [email protected]. , (India)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Dec 09, 2023
Pages
128717–128717
Identifiers
DOI: 10.1016/j.ijbiomac.2023.128717
PMID: 38081485
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Biopolymer-based nanoscale drug delivery systems have become a promising approach to overcome the limitations associated with conventional chemotherapeutics used for cancer treatment. Herein, we reported to develop a hydrophilic nanogel (NG) composed of Chitosan (Chi) and sodium alginate (Alg) using the ion gelation method for delivering Berberine hydrochloride (BBR), an alkaloid obtained from Berberis aristata roots. The use of different nanocarriers for BBR delivery has been reported previously, but the bioavailability of these carriers was limited due to phagocytic uptake and poor systemic delivery. The developed NG showed enhanced stability and efficient entrapment of BBR ~92 %, resulting in a significant increase in bioavailability. The pH-dependent release behavior demonstrated sustained and effective release of ~86 %, ~74 % and, ~53 % BBR at pH 5.5, 6.6, and 7.4 respectively after 72 h, indicating its potential as a drug carrier. Additionally, the cellular uptake of BBR was significantly higher ~19 % in the BBR-NG (25 μM) than in bulk BBR (100 μM), leading to enhanced ROS generation, mitochondrial depolarisation, and inhibition of cell proliferation and colony formation in HepG2 cells. In summary, the results suggest that the Chi/Alg biopolymer-based nano-formulation could be an effective approach for delivering BBR and enhancing its cellular uptake, efficacy, and cytotoxicity. Copyright © 2023. Published by Elsevier B.V.

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