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Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum.

Authors
  • Gopalakrishnan, Subramanium
  • Beale, Michael H
  • Ward, Jane L
  • Strange, Richard N
Type
Published Article
Journal
Phytochemistry
Publisher
Elsevier
Publication Date
Jul 01, 2005
Volume
66
Issue
13
Pages
1536–1539
Identifiers
PMID: 15963542
Source
Medline
License
Unknown

Abstract

Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and lambda(max) at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.

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