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Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6

Authors
  • García Caballero, Gabriel
  • Schmidt, Sebastian
  • Schnölzer, Martina
  • Schlötzer-Schrehardt, Ursula
  • Knospe, Clemens
  • Ludwig, Anna-Kristin
  • Manning, Joachim C.
  • Muschler, Paul
  • Kaltner, Herbert
  • Kopitz, Jürgen
  • Gabius, Hans-Joachim
Type
Published Article
Journal
Cell and Tissue Research
Publisher
Springer-Verlag
Publication Date
Oct 17, 2018
Volume
375
Issue
3
Pages
665–683
Identifiers
DOI: 10.1007/s00441-018-2931-x
Source
Springer Nature
Keywords
License
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Abstract

Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the profile of localization. Having identified the chicken galectin-related interfiber protein (C-GRIFIN) as lens-specific protein present in the main body of adult lens, we here report its interaction with lens proteins in ligand blotting. The assumption for pairing with α-, β- and δ-crystallins was ascertained by mass spectrometric detection of their presence in eluted fractions obtained by affinity chromatography. Biochemical and immunohistochemical monitoring revealed protein presence from about 3-day-old embryos onwards, mostly in the cytoplasm of elongated posterior cells, later in secondary lens fiber cells. On the level of gene expression, its promoter was activated by transcription factor L-Maf alone and together with Pax6 like a crystallin gene, substantiating C-GRIFIN’s status as lens-specific galectin. Using this combined strategy for counterreceptor and expression profiling by bio- and histochemical methods including light, electron and fluorescence microscopy, respective monitoring in lens development can now be taken to the level of the complete galectin family.

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