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The chemorepellent draxin is involved in hippocampal mossy fiber projection.

Authors
  • Tawarayama, Hiroshi1
  • Yamada, Hirohisa2
  • Shinmyo, Yohei2
  • Tanaka, Hideaki2
  • Ikawa, Shuntaro3
  • 1 Department of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan; Department of Project Programs, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai 980-8575, Japan; Department of Ecology and Evolutionary Biology, Graduate School of Life Sciences, Tohoku University, Sendai 980-8578, Japan. Electronic address: [email protected] , (Japan)
  • 2 Department of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan. , (Japan)
  • 3 Department of Project Programs, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai 980-8575, Japan. , (Japan)
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier
Publication Date
Jun 02, 2018
Volume
500
Issue
2
Pages
217–223
Identifiers
DOI: 10.1016/j.bbrc.2018.04.043
PMID: 29634927
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Lamina-specific afferent innervation of the mammalian hippocampus is critical for its function. We investigated the relevance of the chemorepellent draxin to the laminar projections of three principal hippocampal afferents: mossy fibers, entorhinal, and associational/commissural fibers. We observed that draxin deficiency led to abnormal projection of mossy fibers but not other afferents. Immunohistochemical analysis indicated that draxin is expressed in the dentate gyrus and cornu ammonis (CA) 3 at postnatal day 0, when dentate granule cells begin to extend mossy fibers towards CA3. Furthermore, a neurite growth assay using dissociated cells of the neonatal dentate gyrus revealed that draxin inhibited the growth of calbindin-D28k-expressing mossy fibers in vitro. Taken together, we conclude that draxin is a key molecule in the regulation of mossy fiber projections. Copyright © 2018 Elsevier Inc. All rights reserved.

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