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Chemoenzymatic Cascade Reaction for the Valorization of the Lignin Depolymerization Product G-C2-Dioxolane Phenol.

Authors
  • Terholsen, Henrik1
  • Meyer, Jule R H1
  • Zhang, Zhenlei2
  • Deuss, Peter J2
  • Bornscheuer, Uwe T1
  • 1 Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Straße 4, 17487, Greifswald, Germany. , (Germany)
  • 2 Faculty of Science and Engineering, Chemical Technology, Engineering and Technology Institute Groningen, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, Netherlands. , (Netherlands)
Type
Published Article
Journal
ChemSusChem
Publisher
Wiley (John Wiley & Sons)
Publication Date
May 19, 2023
Volume
16
Issue
10
Identifiers
DOI: 10.1002/cssc.202300168
PMID: 36826410
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Combining solid acid catalysts with enzyme reactions in aqueous environments is challenging because either very acidic conditions inactivate the enzymes, or the solid acid catalyst is neutralized. In this study, Amberlyst-15 encapsulated in polydimethylsiloxane (Amb-15@PDMS) is used to deprotect the lignin depolymerization product G-C2 dioxolane phenol in a buffered system at pH 6.0. This reaction is directly coupled with the biocatalytic reduction of the released homovanillin to homovanillyl alcohol by recombinant horse liver alcohol dehydrogenase, which is subsequently acylated by the promiscuous acyltransferase/hydrolase PestE_I208A_L209F_N288A in a one-pot system. The deprotection catalyzed with Amb-15@PDMS attains up to 97 % conversion. Overall, this cascade enables conversions of up to 57 %. © 2023 The Authors. ChemSusChem published by Wiley-VCH GmbH.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. This record was last updated on 05/28/2023 and may not reflect the most current and accurate biomedical/scientific data available from NLM. The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/36826410
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