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Chemical mapping of ceramide distribution in sphingomyelin-rich domains in monolayers.

Authors
  • Popov, Jesse
  • Vobornik, Dusan
  • Coban, Oana
  • Keating, Eleonora
  • Miller, David
  • Francis, James
  • Petersen, Nils O
  • Johnston, Linda J
Type
Published Article
Journal
Langmuir : the ACS journal of surfaces and colloids
Publication Date
Dec 02, 2008
Volume
24
Issue
23
Pages
13502–13508
Identifiers
DOI: 10.1021/la8007552
PMID: 18973350
Source
Medline
License
Unknown

Abstract

The incorporation of ceramide in phase-separated monolayers of ternary lipid mixtures has been studied by a combination of atomic force microscopy (AFM), fluorescence, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Replacement of a fraction of the sphingomyelin by ceramide in DOPC/SM/cholesterol monolayers leads to changes in the SM-cholesterol-rich liquid-ordered domains. AFM shows the formation of heterogeneous domains with small raised islands that are assigned to a ceramide-rich gel phase. ToF-SIMS provides conclusive evidence for the localization of SM and ceramide in ordered domains and shows that ceramide is heterogeneously distributed in small islands throughout the domains. The results indicate the utility of combining AFM and ToF-SIMS for understanding compositions of phase-separated membranes.

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