Aedes aegypti mosquito is the major vector of zika, chikungunya, and dengue fever. These diseases incidence has been growing rapidly in many points of the globe in the past few years. And because there's no vaccine for them yet, the best way to fight those diseases is to attack their vector, specially by eliminating potential sites for its oviposition and larvae growth. Nowadays, organophosphorus insecticides are used in increasing doses, which targets Aedes aegypti resistant populations. Aniba duckei Kostermans, which is known as rosewood and belongs to the Lauraceae family, is a species with trees up to 30 meters tall and 1 meter in trunk diameter. It's essential oil is used in perfumery due to its high content of linalool. This research identified the components of essential oil from Aniba duckei Kostermans thin branches and leaves and then applied it as larvicide against Aedes aegypti, and its effects were measured by calculation of concentration at which half larvae die (LC 50). Average yield found for oil by plant was 1.93% by mass. The major component in rosewood essential oil is linalool, whose concentration was found 89.34% by mass. LC 50 for the essential oil was 250.61 (±2.20) µg mL-1 , for l-linalool, 279.89 (±2.12) µg mL-1 , and for dl-linalool was 346.73 (±2.14) µg mL-1 .