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Charcot-Leyden crystals: do they exist in veterinary species? A case report and literature review.

Authors
  • Choi, Eunju1, 2, 3
  • Miller, Andrew D1, 2, 3
  • Devenish, Elizabeth1, 2, 3
  • Asakawa, Makoto1, 2, 3
  • McConkey, Marina1, 2, 3
  • Peters-Kennedy, Jeanine1, 2, 3
  • 1 Section of Anatomic Pathology, Department of Biomedical Sciences (Choi, Miller, Peters-Kennedy).
  • 2 Section of Small Animal Surgery, Department of Clinical Sciences (Asakawa, McConkey).
  • 3 Cornell University, College of Veterinary Medicine, Ithaca, NY (Devenish).
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Nov 01, 2017
Volume
29
Issue
6
Pages
904–909
Identifiers
DOI: 10.1177/1040638717725783
PMID: 28782436
Source
Medline
Keywords
License
Unknown

Abstract

The Charcot-Leyden crystal (CLC) is a major human eosinophil protein that readily crystallizes; these crystals are common in eosinophilic diseases. Although anecdotal existence of these crystals is known in veterinary pathology, definitive reports do not exist, to our knowledge. We identified eosinophilic crystals in a laryngeal myxosarcoma from a 2-y-old, spayed female, Labrador Retriever dog that were tentatively interpreted as CLCs. However, Ziehl-Neelsen acid-fast stain was negative, arguing against CLCs. The crystals stained red with Masson trichrome, precluding collagen. Periodic acid-Schiff and alcian blue were negative. The crystals stained positively with Okajima, and no myoglobin immunoreactivity was detected, supporting their identity as hemoglobin crystals. In the absence of a hematologic abnormality, these crystals were interpreted to be abnormal hemoglobin breakdown products. Protein sequence comparison was pursued to determine whether a protein similar to CLC exists in mammals. Only 3 nonhuman primate species, the Sumatran orangutan ( Pongo abelii), rhesus macaque ( Macaca mulatta), and cynomolgus monkey ( Macaca fascicularis), had a sequence similarity of >80%. Of the crystal-forming residues, 12 of 54 (22%) were different in the Sumatran orangutan and 15 of 54 (28%) were different in the Macaca spp., which may affect the crystallization process. The lack of reports of CLCs in nonhuman species and our results collectively suggest that CLCs are human-specific.

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