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Characterization of thraustochytrid-specific sterol O-acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186

Authors
  • Ishibashi, Yohei
  • Sadamitsu, Shohei
  • Fukahori, Yoshitomo
  • Yamamoto, Yuki
  • Tanogashira, Rin
  • Watanabe, Takashi
  • Hayashi, Masahiro
  • Ito, Makoto
  • Okino, Nozomu
Type
Published Article
Journal
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Publication Date
Oct 24, 2023
Volume
89
Issue
11
Identifiers
DOI: 10.1128/aem.01001-23
Source
ASM Journals
Keywords
License
Green

Abstract

Since the global market for sterols and vitamin D are grown with a high compound annual growth rate, a sustainable source of these compounds is required to keep up with the increasing demand. Thraustochytrid is a marine oleaginous microorganism that can synthesize several sterols, which are stored as SE in lipid droplets. DGAT2C is an unconventional SE synthase specific to thraustochytrids. Although the primary structure of DGAT2C shows high similarities with that of DGAT, DGAT2C utilizes sterol as an acceptor substrate instead of diacylglycerol. In this study, we examined more detailed enzymatic properties, intracellular localization, and structure-activity relationship of DGAT2C. Furthermore, we successfully developed a method to increase sterol and provitamin D3 productivity of thraustochytrid by more than threefold in the process of elucidating the function of the DGAT2C-specific N-terminal region. Our findings could lead to sustainable sterol and vitamin D production using thraustochytrid.

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