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Characterization of selected gram-positive bacteria of the human periodontal region by capillary gas chromatography of cellular carbohydrates and by DNA hybridization Final report / Charakterisierung ausgewaehlter gram-positiver Bakterien aus dem Paradontalbereich mittels kapillargaschromatographisch-massenspektrometrischer Analyse ihrer Kohlenhydrate und durch DNA-Hybridisierung Schlussbericht

Authors
  • Mutters, R.
  • univ., marburg
Publication Date
Jan 01, 1997
Source
OpenGrey Repository
Keywords
Language
German
License
Unknown

Abstract

The taxonomy of anaerbobic gram-positive bacteria of the genera Peptostreptococcus, Actinomyces and Eubacterium is mostly undetermined, the identification of species with the existing methods time-consuming and difficult. By DNA:DNA hybridisation and analysis of cellular carbohydrates the classification of these organisms possibly could be improved. Additionally it should be scrutinized if analysis of carbohydrates was useful for the identification of these gram-positive anaerobic species. It could be shown that peptostreptococci resemble a true genus but within the genus several species have to be reclassified. Also Actinomyces is a true genus, the species within the genus are related on a higher taxonomic level as known from gram-negative bacteria. On the basis of own data the genus Eubacterium proved to be false classified. Therefore more investigations are necessary to decide which species can be included in a true genus Eubacterium. In the case of members of the genus Peptostreptococcus the cellular carbohydrates were useful for identification and separation of species. In the case of Actinomyces more criteria have to be used. With Eubacterium extensive genetic examinations are badly needed to provide a base for further examinations. At the present state no decision of the suitability of carbohydrate analysis can be made. (orig.) / SIGLE / Available from TIB Hannover: DtF QN1(66,48) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische Informationsbibliothek / Bundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany) / DE / Germany

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