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Characterization of a rice gene coding for a lipid transfer protein.

Authors
  • Vignols, F
  • Lund, G
  • Pammi, S
  • Trémousaygue, D
  • Grellet, F
  • Kader, J C
  • Puigdomènech, P
  • Delseny, M
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
May 16, 1994
Volume
142
Issue
2
Pages
265–270
Identifiers
PMID: 8194762
Source
Medline
License
Unknown

Abstract

The cloning and sequence analysis of a gene that encodes a lipid transfer protein (LTP) from rice is reported. A genomic DNA library from Oryza sativa was screened using a cDNA encoding a maize LTP. One genomic clone containing the gene (Ltp) was partially sequenced and analyzed. The open reading frame is interrupted by an 89-bp intron. From the results of Southern hybridizations, Ltp appears to be a member of a small multigenic family. Transcripts of the corresponding gene were detected in several tissues including coleoptile, leaf, endosperm, scutellum and root. The transcription start point was determined by primer extension. The deduced amino-acid sequence of the Ltp product is shown to be homologous to LTPs from other crops.

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