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Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed β-propeller fold.

Authors
  • Sýkorová, Petra1
  • Novotná, Jitka2
  • Demo, Gabriel3
  • Pompidor, Guillaume4
  • Dubská, Eva5
  • Komárek, Jan2
  • Fujdiarová, Eva2
  • Houser, Josef2
  • Hároníková, Lucia6
  • Varrot, Annabelle7
  • Shilova, Nadezhda8
  • Imberty, Anne7
  • Bovin, Nicolai8
  • Pokorná, Martina2
  • Wimmerová, Michaela9
  • 1 Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Masaryk University, Brno, Czech Republic. , (Czechia)
  • 2 Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic. , (Czechia)
  • 3 Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic; current address: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA. , (Czechia)
  • 4 EMBL Hamburg c/o DESY, Hamburg, Germany. , (Germany)
  • 5 Central European Institute of Technology, Masaryk University, Brno, Czech Republic. , (Czechia)
  • 6 Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic. , (Czechia)
  • 7 CERMAV, CNRS Université de Grenoble Alpes, Grenoble, France. , (France)
  • 8 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. , (Russia)
  • 9 Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Nov 18, 2019
Identifiers
DOI: 10.1016/j.ijbiomac.2019.10.200
PMID: 31751748
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria - BP39L and CV39L. The crystal structures revealed that the lectins possess a seven-bladed β-propeller fold. Functional studies conducted on a series of oligo- and polysaccharides confirmed the preference of BP39L for mannosylated saccharides and CV39L for rather more complex polysaccharides with a monosaccharide preference for β-L-fucose. The presented data indicate that the proteins belong to a currently unknown family of lectins. Copyright © 2019. Published by Elsevier B.V.

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