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Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China

Authors
  • Zheng, Kuanyu1
  • Chen, Tsung-Chi2, 3
  • Yeh, Shyi-Dong4
  • Rahman, Md. Siddiqur1, 5
  • Su, Xiaoxia1
  • Wu, Kuo1
  • Li, Ting1
  • Zhang, Zhongkai1
  • Dong, Jiahong1
  • 1 Yunnan Academy of Agricultural Sciences. Yunnan Provincial Key Lab of Agricultural Biotechnology. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Biotechnology and Germplasm Resources Institute, 9# Xueyun Rd, Wuhua Prefecture, Kunming, Yunnan, 650223, People’s Republic of China , Kunming (China)
  • 2 Asia University, Department of Biotechnology, Wufeng, Taichung, 41354, Taiwan , Taichung (Taiwan)
  • 3 China Medical University, Department of Medical Research, China Medical University Hospital, Taichung, 40402, Taiwan , Taichung (Taiwan)
  • 4 National Chung Hsing University, Department of Plant Pathology, Taichung, 402, Taiwan , Taichung (Taiwan)
  • 5 Bangladesh Agricultural Research Institute, Gazipur, 1701, Bangladesh , Gazipur (Bangladesh)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
May 19, 2017
Volume
162
Issue
9
Pages
2809–2814
Identifiers
DOI: 10.1007/s00705-017-3402-5
Source
Springer Nature
Keywords
License
Yellow

Abstract

A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80–120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively. This is the first report of complete sequences of PCSV in mainland China. Phylogenetic analysis of all tospoviral proteins indicated that PCSV-14YV733 is closely related to members of the WSMoV serogroup.

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