Affordable Access

Characterization of natural hybrids of Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum.

Authors
  • Le Jeune, Christine1
  • Lollier, Marc
  • Demuyter, Catherine
  • Erny, Claude
  • Legras, Jean-Luc
  • Aigle, Michel
  • Masneuf-Pomarède, Isabelle
  • 1 Laboratoire Vigne Biotechnologie et Environnement, Université de Haute-Alsace, Herrlisheim, Colmar, France. [email protected] , (France)
Type
Published Article
Journal
FEMS yeast research
Publication Date
Jun 01, 2007
Volume
7
Issue
4
Pages
540–549
Identifiers
PMID: 17302940
Source
Medline
Language
English
License
Unknown

Abstract

Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Karyotypes and MET2 fragments of the nine strains corresponded to mixed chromosomal bands and restriction patterns for both Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. They also responded positively to amplification with microsatellite primers specific to both species and were demonstrated to be diploid. However, meiosis led to absolute nonviability of their spores on complete medium. All the results demonstrated that the nine yeast strains isolated were S. cerevisiaexS. bayanus var. uvarum diploid hybrids. Moreover, microsatellite DNA analysis identified strains isolated in the same cellar as potential parents belonging to S. bayanus var. uvarum and S. cerevisiae.

Report this publication

Statistics

Seen <100 times