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Characterization of macrophage cytotoxicity factor (MCF).

Authors
Type
Published Article
Journal
International archives of allergy and applied immunology
Publication Date
Volume
53
Issue
1
Pages
37–41
Identifiers
PMID: 300074
Source
Medline

Abstract

Allogenetically sensitized T lymphocytes secrete in the presence of the specific antigen a factor which renders macrophages cytotoxic to target cells in vitro, macrophage cytotoxicity factor. Cytotoxicity is expressed as 51Cr release from labeled target cells. Experiments are described to characterize chemically nonspecific MCF activity. After purification on a DEAE-cellulose column, active material elutes from Sephadex G-100 in a single peak. Assaying individual fractions of this peak shows that nonspecific MCF activity is found at a mol. wt. of 28,000+/-5,000. This material is stable at -70 degrees C several months.

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