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Characterization of M cell formation and associated mononuclear cells during indomethacin-induced intestinal inflammation.

Authors
  • Lügering, A
  • Floer, M
  • Lügering, N
  • Cichon, C
  • Schmidt, M A
  • Domschke, W
  • Kucharzik, T
Type
Published Article
Journal
Clinical and experimental immunology
Publication Date
May 01, 2004
Volume
136
Issue
2
Pages
232–238
Identifiers
PMID: 15086385
Source
Medline
License
Unknown

Abstract

M cells represent an important gateway for the intestinal immune system by delivering luminal antigens through the follicle-associated epithelium to the underlying immune cells. The goal of this study was to characterize this route of antigen uptake during intestinal inflammation by characterizing M cell formation and M cell-associated lymphocytes after indomethacin challenge in rats. We demonstrated increased M cell formation as early as 12 h after a single injection of indomethacin. The elevated M cell counts were determined until day 3 and returned to basal levels after 7 days. Electron microscopic studies revealed an expansion of mononuclear cells inside the M cell pocket that were characterized predominantly as B cells, T cell receptor (TCR)alphabeta- and CD4-positive T cells, whereas other markers such as CD11b, CD8 and CD25 remained unchanged. In situ hybridization studies showed increased expression of interleukin (IL)-4 by lymphocytes during intestinal inflammation in the Peyer's patch follicle. These studies illuminate the relevance of M cells during intestinal inflammation and suggest that M cells derive from epithelial cells in a certain microenvironment.

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