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Characterization of a Lipase From the Silkworm Intestinal Bacterium Bacillus pumilus With Antiviral Activity Against Bombyx mori (Lepidoptera: Bombycidae) Nucleopolyhedrovirus In Vitro.

Authors
  • Liu, Renhua1, 2
  • Wang, Wenhui1
  • Liu, Xiaoyuan1
  • Lu, Yan1
  • Xiang, Tingting1
  • Zhou, Wei1
  • Wan, Yongji1
  • 1 Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, China. , (China)
  • 2 School of Biological and Food Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing, China. , (China)
Type
Published Article
Journal
Journal of Insect Science
Publisher
Oxford University Press
Publication Date
Nov 01, 2018
Volume
18
Issue
6
Identifiers
DOI: 10.1093/jisesa/iey111
PMID: 30395292
Source
Medline
Language
English
License
Unknown

Abstract

To investigate whether Bombyx mori Linnaeus (Lepidoptera: Bombycidae) intestinal microorganism play a role in the host defence system against viral pathogens, a lipase gene from the silkworm intestinal bacterium Bacillus pumilus SW41 was characterized, and antiviral activity of its protein against B. mori nucleopolyhedrovirus (BmNPV) was tested. The lipase gene has an open-reading frame of 648 bp, which encodes a 215-amino-acid enzyme with a 34-amino-acid signal peptide. The recombinant lipase (without signal peptide) was expressed and purified by using an Escherichia coli BL21 (DE3) expression system. The total enzyme activity of this recombinant lipase reached 277.40 U/mg at the optimum temperature of 25°C and optimum pH value of 8.0. The antiviral test showed that a relative high concentration of the recombinant lipase reduced BmNPV infectivity in vitro, which resulted in decreased viral DNA abundance and viral occlusion bodies. Besides, the preincubation method also suggested that the lipase probably directly acting on the budded virions. The results suggest that the lipase from intestinal bacterium B. pumilus SW41 is a potential antiviral factor for silkworm against BmNPV.

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