The aim of this study is to design and test a new high-resolution hybrid depth of interaction (DOI) detector for a dedicated breast PET/CT scanner. Two detectors have been designed and built. The completed detectors are based on a 14 × 14 array of 1.5 × 1.5 × 20 mm(3) unpolished lutetium orthosilicate scintillation crystals, with each element coated in a 50 μm layer of reflective material. The detector is read out from both ends using a position-sensitive photomultiplier tube (PSPMT) and a large active area (20 × 20 mm(2)) avalanche photodiode (APD) to enable acquisition of DOI information. Nuclear instrumentation modules were used to characterize the detectors performances in terms of timing, intrinsic spatial resolution (ISR) and energy resolution, as well as DOI resolution with a dual-ended readout configuration. Measurements with the APD were performed at a temperature of 10 °C. All crystals were identified at all depths, even though the signal amplitude from the PSPMT decreases with depth away from it. We measured a timing resolution of 2.4 ns, and an average energy resolution of 19%. The mean ISR was measured to be 1.2 mm for crystals in the central row of the array for detectors in the face-to-face position. Two off-center positions were measured corresponding to 26° and 51° oblique photon incidence, and the mean ISR at these positions was 1.5 and 1.7 mm, respectively. The average DOI resolution across all crystals and depths was measured to be 2.9 mm (including the beam width of 0.6 mm). This detector design shows good promise as a high-resolution detector for a dedicated breast PET/CT scanner.