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Characterization of a heme-dependent catalase from Methanobrevibacter arboriphilus.

Authors
  • Shima, S
  • Sordel-Klippert, M
  • Brioukhanov, A
  • Netrusov, A
  • Linder, D
  • Thauer, R K
Type
Published Article
Journal
Applied and environmental microbiology
Publication Date
Jul 01, 2001
Volume
67
Issue
7
Pages
3041–3045
Identifiers
PMID: 11425719
Source
Medline
License
Unknown

Abstract

Recently it was reported that methanogens of the genus Methanobrevibacter exhibit catalase activity. This was surprising, since Methanobrevibacter species belong to the order Methanobacteriales, which are known not to contain cytochromes and to lack the ability to synthesize heme. We report here that Methanobrevibacter arboriphilus strains AZ and DH1 contained catalase activity only when the growth medium was supplemented with hemin. The heme catalase was purified and characterized, and the encoding gene was cloned. The amino acid sequence of the catalase from the methanogens is most similar to that of Methanosarcina barkeri.

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